Metabolic fluxes in the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis, two members of the marine Roseobacter clade.

ABSTRACT:

Background

In the present work the central carbon metabolism of Dinoroseobacter shibae and Phaeobacter gallaeciensis was studied at the level of metabolic fluxes. These two strains belong to the marine Roseobacter clade, a dominant bacterial group in various marine habitats, and represent surface-associated, biofilm-forming growth (P. gallaeciensis) and symbiotic growth with eukaryotic algae (D. shibae). Based on information from recently sequenced genomes, a rich repertoire of pathways has been identified in the carbon core metabolism of these organisms, but little is known about the actual contribution of the various reactions in vivo.

Results

Using 13C labelling techniques in specifically designed experiments, it could be shown that glucose-grown cells of D. shibae catabo

SUBMITTER: Furch T 

PROVIDER: S-EPMC2761930 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature