Structural characterization of unsaturated phosphatidylcholines using traveling wave ion mobility spectrometry.

ABSTRACT: A number of phosphatidylcholine (PC) cations spanning a mass range of 400-1000 Da are investigated using electrospray ionization mass spectrometry coupled with traveling wave ion mobility spectrometry (TWIMS). A high correlation between mass and mobility is demonstrated with saturated phosphatidylcholine cations in N(2). A significant deviation from this mass-mobility correlation line is observed for the unsaturated PC cation. We found that the double bond in the acyl chain causes a 5% reduction in drift time. The drift time is reduced at a rate of approximately 1% for each additional double bond. Theoretical collision cross sections of PC cations exhibit good agreement with experimentally evaluated values. Collision cross sections are determined using the recently derived relationship bet

SUBMITTER: Kim HI 

PROVIDER: S-EPMC2761977 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature