Slow intracellular accumulation of GABA(A) receptor delta subunit is modulated by brain-derived neurotrophic factor.

ABSTRACT: GABA(A) receptors composed of the gamma2 and delta subunits have distinct properties, functions and subcellular localization, and pathological conditions differentially modulate their surface expression. Recent studies demonstrate that acute seizure activity accelerated trafficking of the gamma2 and beta2/3 subunits but not that of the delta subunit. The trafficking of the gamma2 and beta2/3 subunits is relatively well understood but that of the delta subunit has not been studied. We compared intracellular accumulation of the delta and gamma2 subunits in cultured hippocampal neurons using an antibody feeding technique. Intracellular accumulation of the delta subunit peaked between 3 and 6 h, whereas, maximum internalization of the gamma2 subunit took 30 min. In the organotypic hippocampal

SUBMITTER: Joshi S 

PROVIDER: S-EPMC2761981 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature