Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers.

ABSTRACT:

Background

The optic nerve is a pure white matter central nervous system (CNS) tract with an isolated blood supply, and is widely used in physiological studies of white matter response to various insults. We examined the gene expression profile of human optic nerve (ON) and, through the NEIBANK online resource, to provide a resource of sequenced verified cDNA clones. An un-normalized cDNA library was constructed from pooled human ON tissues and was used in expressed sequence tag (EST) analysis. Location of an abundant oligodendrocyte marker was examined by immunofluorescence. Quantitative real time polymerase chain reaction (qRT-PCR) and Western analysis were used to compare levels of expression for key calcium channel protein genes and protein product in primate and rodent ON.

SUBMITTER: Bernstein SL 

PROVIDER: S-EPMC2762980 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature