Evaluation of procedures for outer membrane isolation from Campylobacter jejuni.

ABSTRACT: Although infection with Campylobacter jejuni is one of the leading causes of gastroenteritis worldwide, relatively little is known about the factors that are required to elicit a protective immune response. The need for a vaccine against this pathogen is well recognized and a number of vaccine candidates have been tested with varying degrees of success; however, there is still a lack of a suitable vaccine. To gain a better understanding of the outer-membrane protein components of this organism, a 'gold standard' method to purify the outer membrane is needed. Therefore, we attempted to develop a robust and reliable method which resulted in a pure outer-membrane fraction. A total of nine methodologies were examined and analysed by SDS-PAGE and immunoblotting using subcellular markers for the

SUBMITTER: Hobb RI 

PROVIDER: S-EPMC2763183 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature