E Unibus Plurum: genomic analysis of an experimentally evolved polymorphism in Escherichia coli.

ABSTRACT: Microbial populations founded by a single clone and propagated under resource limitation can become polymorphic. We sought to elucidate genetic mechanisms whereby a polymorphism evolved in Escherichia coli under glucose limitation and persisted because of cross-feeding among multiple adaptive clones. Apart from a 29 kb deletion in the dominant clone, no large-scale genomic changes distinguished evolved clones from their common ancestor. Using transcriptional profiling on co-evolved clones cultured separately under glucose-limitation we identified 180 genes significantly altered in expression relative to the common ancestor grown under similar conditions. Ninety of these were similarly expressed in all clones, and many of the genes affected (e.g., mglBAC, mglD, and lamB) are in operons coor

SUBMITTER: Kinnersley MA 

PROVIDER: S-EPMC2763269 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature