Silencing of genes required for glycosylphosphatidylinositol anchor biosynthesis in Burkitt lymphoma.

ABSTRACT:

Objective

To investigate the mechanism of glycosylphosphatidylinositol (GPI) anchor deficiency in Burkitt lymphoma cell lines.

Methods

We identified a large GPI anchor protein deficient population in three different Burkitt lymphoma cell lines through proaerolysin treatment of the cells and flow cytometry analysis using a proaerolysin variant (FLAER). The mechanism of GPI anchor protein deficiency was studied by DNA gene sequencing, a cell-free assay to investigate the GPI anchor biosynthetic pathway, microarray analysis, and quantitative real-time polymerase chain reaction.

Results

Burkitt lymphoma cell lines harbor large populations of FLAER(neg) cells, which are resistant to proaerolysin. In all three cell lines, silencing of a gene involved in an early step in GPI

SUBMITTER: Hu R 

PROVIDER: S-EPMC2763298 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature