Microarray analysis of prothrombin knockdown in zebrafish.

ABSTRACT: The serine protease thrombin is generated from its precursor, prothrombin, in the coagulation cascade and plays a central role in fibrin deposition and platelet activation mediated through the protease activated receptors. Knockdown of prothrombin in the zebrafish was previously shown to recapitulate the phenotype observed in prothrombin knockout mice, such as an absence of blood pericardial edema, and hemorrhage. However, the role of thrombin during embryogenesis is not fully understood. To find genes affected by potential thrombin signaling in embryogenesis before blood circulation, microarray analysis was performed using total RNA prepared from antisense-injected, knockdown embryos versus mismatch-injected at 20 h post fertilization. A total of 63 upregulated and downregulated genes wer

SUBMITTER: Day KR 

PROVIDER: S-EPMC2763619 | biostudies-literature | 2009 Sep-Oct

REPOSITORIES: biostudies-literature