A stable isotope method for the simultaneous measurement of matrix synthesis and cell proliferation in articular cartilage in vivo.

ABSTRACT:

Objective

Measurements of cell proliferation and matrix synthesis in cartilage explants have identified regulatory factors [e.g., interleukin-1 (IL-1)] that contribute to osteoarthritis and anabolic mediators [e.g., bone morphogenic protein-7 (BMP-7)] that may have therapeutic potential. The objective of this study was to develop a robust method for measuring cell proliferation and glycosaminoglycan synthesis in articular cartilage that could be applied in vivo.

Methods

A stable isotope-mass spectrometry approach was validated by measuring the metabolic effects of IL-1 and BMP-7 in cultures of mature and immature bovine cartilage explants. The method was also applied in vivo to quantify physiologic turnover rates of matrix and cells in the articular cartilage of normal rats.

SUBMITTER: Li KW 

PROVIDER: S-EPMC2763636 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature