Implementation of two high through-put techniques in a novel application: detecting point mutations in large EMS mutated plant populations.

ABSTRACT:

Background

The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Considerable efforts have been invested into research on tomato as a model for berry-fruit plants. With the progress of the tomato sequencing project, reverse genetics becomes an obvious and achievable goal.

Results

Here we describe the treatment of Solanum lycopersicum seeds with 1% EMS and the development of a new mutated tomato population. To increase targeted mutant detection throughput an automated seed DNA extraction has been combined with novel mutation detection platforms for TILLING in plants. We have adapted two techniques used in human genetic diagno

SUBMITTER: Gady AL 

PROVIDER: S-EPMC2763861 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature