Global characterization of Artemisia annua glandular trichome transcriptome using 454 pyrosequencing.

ABSTRACT:

Background

Glandular trichomes produce a wide variety of commercially important secondary metabolites in many plant species. The most prominent anti-malarial drug artemisinin, a sesquiterpene lactone, is produced in glandular trichomes of Artemisia annua. However, only limited genomic information is currently available in this non-model plant species.

Results

We present a global characterization of A. annua glandular trichome transcriptome using 454 pyrosequencing. Sequencing runs using two normalized cDNA collections from glandular trichomes yielded 406,044 expressed sequence tags (average length = 210 nucleotides), which assembled into 42,678 contigs and 147,699 singletons. Performing a second sequencing run only increased the number of genes identified by approximately 30

SUBMITTER: Wang W 

PROVIDER: S-EPMC2763888 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature