FoxO4 inhibits NF-kappaB and protects mice against colonic injury and inflammation.

ABSTRACT:

Background & aims

FoxO4 is a member of the forkhead box transcription factor O (FoxO) subfamily. FoxO proteins are involved in diverse biological processes. In this study, we examine the role of FoxO4 in intestinal mucosal immunity and inflammatory bowel disease (IBD).

Methods

Foxo4-null mice were subjected to trinitrobenzene sulfonic acid (TNBS) treatment. Microarray analysis and quantitative reverse transcription polymerase chain reaction were used to identify the cytokine transcripts that were altered by Foxo4 deletion. The effects of Foxo4 deficiency on the intestinal epithelial permeability and levels of tight junction proteins were examined by permeable fluorescent dye and Western blot. The molecular and cellular mechanisms by which FoxO4 regulates the mucosal immunity

SUBMITTER: Zhou W 

PROVIDER: S-EPMC2764529 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature