Spectroelectrochemical determination of the redox potential of pheophytin a, the primary electron acceptor in photosystem II.

ABSTRACT: Thin-layer cell spectroelectrochemistry, featuring rigorous potential control and rapid redox equilibration within the cell, was used to measure the redox potential E(m)(Phe a/Phe a(-)) of pheophytin (Phe) a, the primary electron acceptor in an oxygen-evolving photosystem (PS) II core complex from a thermophilic cyanobacterium Thermosynechococcus elongatus. Interferences from dissolved O(2) and water reductions were minimized by airtight sealing of the sample cell added with dithionite and mercury plating on the gold minigrid working electrode surface, respectively. The result obtained at a physiological pH of 6.5 was E(m)(Phe a/Phe a(-)) = -505 + or - 6 mV vs. SHE, which is by approximately 100 mV more positive than the values measured approximately 30 years ago at nonphysiological pH and

SUBMITTER: Kato Y 

PROVIDER: S-EPMC2765088 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature