Sequential changes in the gene expression profile of murine retinal progenitor cells during the induction of differentiation.

ABSTRACT:

Purpose

Following transplantation, cultured retinal progenitor cells (RPCs) integrate into the diseased host retina and exhibit morphologies and markers indicative of local cellular phenotypes. In vitro analysis of cultured RPCs allows detailed examination of marker gene expression during the initial phase of differentiation and can provide insight into the variables influencing this process.

Methods

Using cultured murine RPCs, this study compares the effects of fetal bovine serum (FBS) with those of ciliary neurotrophic factor (CNTF), individually or in combination with epidermal growth factor (EGF). Differentiation was assessed by way of the relative expression of 17 genes using quantitative PCR (qPCR) at five time points over a seven-day period.

Results

Both CNTF a

SUBMITTER: Gu P 

PROVIDER: S-EPMC2765240 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature