ETX1 is over-expressed in the glaucomatous trabecular meshwork.

ABSTRACT:

Purpose

To determine whether exon-trapped X chromosome clone 1 (ETX1) is overexpressed in the trabecular meshwork (TM) of glaucomatous human eyes compared to controls.

Methods

Immunohistochemical, western blot, and enzyme-linked immunosorbent assay analysis were used with human tissues and TM protein extracts. Reverse transcription-PCR was performed on isolated mRNA-derived cDNA preparations.

Results

Elevated expression levels of ETX1 were detected in glaucomatous compared to control TM tissue. This corroborates previous detection of ETX1 in glaucomatous TM by proteomic analysis. ETX1 mRNA is present in TM tissue, suggesting ETX1 protein is locally produced within TM cells.

Conclusions

This is the first report demonstrating overexpression of ETX1 in glaucomatou

SUBMITTER: Iragavarapu S 

PROVIDER: S-EPMC2765241 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature