High-potential C112D/M121X (X = M, E, H, L) Pseudomonas aeruginosa azurins.

ABSTRACT: Site-directed mutagenesis of Pseudomonas aeruginosa azurin C112D at the M121 position has afforded a series of proteins with elevated Cu(II/I) reduction potentials relative to the Cu(II) aquo ion. The high potential and low axial hyperfine splitting (Cu(II) electron paramagnetic resonance A( parallel)) of the C112D/M121L protein are remarkably similar to features normally associated with type 1 copper centers.

SUBMITTER: Lancaster KM 

PROVIDER: S-EPMC2765487 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature