Fiber-optic microsphere-based antibody array for the analysis of inflammatory cytokines in saliva.

ABSTRACT: Antibody microarrays have emerged as useful tools for high-throughput protein analysis and candidate biomarker screening. We describe here the development of a multiplexed microsphere-based antibody array capable of simultaneously measuring 10 inflammatory protein mediators. Cytokine-capture microspheres were fabricated by covalently coupling monoclonal antibodies specific for cytokines of interest to fluorescently encoded 3.1 microm polymer microspheres. An optical fiber bundle containing approximately 50,000 individual 3.1 microm diameter fibers was chemically etched to create microwells in which cytokine-capture microspheres could be deposited. Microspheres were randomly distributed in the wells to produce an antibody array for performing a multiplexed sandwich immunoassay. The array re

SUBMITTER: Blicharz TM 

PROVIDER: S-EPMC2765577 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature