Developmental and genetic regulation of human surfactant protein B in vivo.

ABSTRACT:

Background

Genetic and developmental disruption of surfactant protein B (SP-B) expression causes neonatal respiratory distress syndrome (RDS).

Objectives

To assess developmental and genetic regulation of SP-B expression in vivo.

Methods

To evaluate in vivo developmental regulation of SP-B, we used immunoblotting to compare frequency of detection of mature and pro-SP-B peptides in developmentally distinct cohorts: 24 amniotic fluid samples, unfractionated tracheal aspirates from 101 infants >or=34 weeks' gestation with (75) and without (26) neonatal RDS, and 6 nonsmoking adults. To examine genetic regulation, we used univariate and logistic regression analyses to detect associations between common SP-B (SFTPB) genotypes and SP-B peptides in the neonatal RDS cohort.

SUBMITTER: Hamvas A 

PROVIDER: S-EPMC2765709 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature