A tamoxifen inducible knock-in allele for investigation of E2A function.

ABSTRACT:

Background

E-proteins are transcription factors important for the development of a variety of cell types, including neural, muscle and lymphocytes of the immune system. E2A, the best characterized E-protein family member in mammals, has been shown to have stage specific roles in cell differentiation, lineage commitment, proliferation, and survival. However, due to the complexity of E2A function, it is often difficult to separate these roles using conventional genetic approaches. Here, we have developed a new genetic model for reversible control of E2A protein activity at physiological levels. This system was created by inserting a tamoxifen-responsive region of the estrogen receptor (ER) at the carboxyl end of the tcfe2a gene to generate E2AER fusion proteins. We have characterized

SUBMITTER: Jones ME 

PROVIDER: S-EPMC2765948 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature