Flow activation of AMP-activated protein kinase in vascular endothelium leads to Kruppel-like factor 2 expression.

ABSTRACT:

Objective

Vascular endothelial cells (ECs) confer atheroprotection at locations of the arterial tree where pulsatile laminar flow (PS) exists with a high shear stress and a large net forward direction. We investigated whether the PS-induced expression of the transcription factor Krüppel-Like Factor 2 (KLF2) in cultured ECs and its expression in the mouse aorta is regulated by AMP-activated protein kinase (AMPK).

Methods and results

AMPK inhibition by Compound C or siRNA had a significant blocking effect on the PS-induced KLF2 expression. The induction of KLF2 by PS led to the increase in eNOS and the suppression of ET-1, which could be reversed by KLF2 siRNA. In addition, PS induced the phosphorylation of ERK5 and MEF2 which are necessary for the KLF2 expression. These mecha

SUBMITTER: Young A 

PROVIDER: S-EPMC2766008 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature