Real-time imaging and tuning subcellular structures and membrane transport kinetics of single live cells at nanosecond regime.

ABSTRACT: We developed an electric-field exposure microchannel system with 230-nm thin-layer gold electrodes and interfaced it with a single living cell imaging station and a 10-ns electric-pulse (10 nsEP) generator. This design allows us to image intracellular molecules and structures, membrane transport, and viability of single leukemic cells (HL60) while the cells are exposed to 10 nsEPs of 0-179 kV/cm, permitting the study of subcellular responses within a nanosecond regime. The electrodes confine a thin-layer section of the cells exposed to 10 nsEPs, offering unprecedented high spatial resolution (230 nm in the z-direction of imaging plane and electric field) for imaging intracellular molecules of single cells affected by 10 nsEPs. We found that nucleic acids, membrane transport rates, and viab

SUBMITTER: Xu H 

PROVIDER: S-EPMC2766023 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature