Concordant regulation of translation and mRNA abundance for hundreds of targets of a human microRNA.

ABSTRACT: MicroRNAs (miRNAs) regulate gene expression posttranscriptionally by interfering with a target mRNA's translation, stability, or both. We sought to dissect the respective contributions of translational inhibition and mRNA decay to microRNA regulation. We identified direct targets of a specific miRNA, miR-124, by virtue of their association with Argonaute proteins, core components of miRNA effector complexes, in response to miR-124 transfection in human tissue culture cells. In parallel, we assessed mRNA levels and obtained translation profiles using a novel global approach to analyze polysomes separated on sucrose gradients. Analysis of translation profiles for approximately 8,000 genes in these proliferative human cells revealed that basic features of translation are similar to those prev

SUBMITTER: Hendrickson DG 

PROVIDER: S-EPMC2766070 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature