S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility.

ABSTRACT:

Background

High levels of S100A6 have been associated with poor outcome in pancreatic cancer patients. The functional role of S100A6 is, however, poorly understood.

Methods

Immunoprecipitation followed by two-dimensional gel electrophoresis and mass spectrometry were undertaken to identify S100A6 interacting proteins in pancreatic cancer cells. Immunohistochemistry and coimmunofluorescence were performed to examine expression or colocalisation of proteins. siRNA was used to deplete specific proteins and effects on motility were measured using Boyden Chamber and wound healing assays.

Results

Our proteomic screen to identify S100A6 interacting proteins revealed annexin 11, annexin 2, tropomyosin beta and a candidate novel interactor lamin B1. Of these, annexin 2 was con

SUBMITTER: Nedjadi T 

PROVIDER: S-EPMC2768105 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature