Validity and reproducibility of measurement of islet autoreactivity by T-cell assays in subjects with early type 1 diabetes.

ABSTRACT:

Objective

Type 1 diabetes results from an immunemediated destruction of beta-cells, likely to be mediated by T lymphocytes, but the sensitivity, specificity, and other measures of validity of existing assays for islet autoreactive T-cells are not well established. Such assays are vital for monitoring responses to interventions that may modulate disease progression.

Research design and methods

We studied the ability of cellular assays to discriminate responses in patients with type 1 diabetes and normal control subjects in a randomized blinded study in the U.S. and U.K. We evaluated the reproducibility of these measurements overall and to individual analytes from repeat collections.

Results

Responses in the cellular immunoblot, U.K.-ELISPOT, and T-cell proliferation as

SUBMITTER: Herold KC 

PROVIDER: S-EPMC2768166 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature