Hypermetabolism, hyperphagia, and reduced adiposity in tankyrase-deficient mice.

ABSTRACT:

Objective

Tankyrase (TNKS) is a Golgi-associated poly-ADP-ribose polymerase that is implicated in the regulation of GLUT4 trafficking in 3T3-L1 adipocytes. Its chromosomal locus 8p23.1 is linked to monogenic forms of diabetes in certain kindred. We hypothesize that TNKS is involved in energy homeostasis in mammals.

Research design and methods

Gene-trap techniques were used to ablate TNKS expression in mice. Homozygous and wild-type littermates maintained on standard chow were compared.

Results

Wild-type mice express the TNKS protein abundantly in adipose tissue, the brain, and the endocrine pancreas but scarcely in the exocrine pancreas and skeletal muscle. TNKS-deficient mice consume increased amounts of food (by 34%) but have decreased plasma leptin levels and a >50

SUBMITTER: Yeh TY 

PROVIDER: S-EPMC2768175 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature