High resolution analysis of the human transcriptome: detection of extensive alternative splicing independent of transcriptional activity.

ABSTRACT:

Background

Commercially available microarrays have been used in many settings to generate expression profiles for a variety of applications, including target selection for disease detection, classification, profiling for pharmacogenomic response to therapeutics, and potential disease staging. However, many commercially available microarray platforms fail to capture transcript diversity produced by alternative splicing, a major mechanism for driving proteomic diversity through transcript heterogeneity.

Results

The human Genome-Wide SpliceArray(TM) (GWSA), a novel microarray platform, utilizes an existing probe design concept to monitor such transcript diversity on a genome scale. The human GWSA allows the detection of alternatively spliced events within the human genome throu

SUBMITTER: Zhou W 

PROVIDER: S-EPMC2768739 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature