In vitro culture and characterization of a mammary epithelial cell line from Chinese Holstein dairy cow.

ABSTRACT:

Background

The objective of this study was to establish a culture system and elucidate the unique characteristics of a bovine mammary epithelial cell line in vitro.

Methodology

Mammary tissue from a three year old lactating dairy cow (ca. 100 d relative to parturition) was used as a source of the epithelial cell line, which was cultured in collagen-coated tissue culture dishes. Fibroblasts and epithelial cells successively grew and extended from the culturing mammary tissue at the third day. Pure epithelial cells were obtained by passages culture.

Principal findings

The strong positive immunostaining to cytokeratin 18 suggested that the resulting cell line exhibited the specific character of epithelial cells. Epithelial cells cultured in the presence of 10% FBS, supra

SUBMITTER: Hu H 

PROVIDER: S-EPMC2768788 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature