Many LINE1 elements contribute to the transcriptome of human somatic cells.

ABSTRACT:

Background

While LINE1 (L1) retroelements comprise nearly 20% of the human genome, the majority are thought to have been rendered transcriptionally inactive, due to either mutation or epigenetic suppression. How many L1 elements 'escape' these forms of repression and contribute to the transcriptome of human somatic cells? We have cloned out expressed sequence tags corresponding to the 5' and 3' flanks of L1 elements in order to characterize the population of elements that are being actively transcribed. We also examined expression of a select number of elements in different individuals.

Results

We isolated expressed sequence tags from human lymphoblastoid cell lines corresponding to 692 distinct L1 element sites, including 410 full-length elements. Four of the expression tag

SUBMITTER: Rangwala SH 

PROVIDER: S-EPMC2768975 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature