Networking of differentially expressed genes in human cancer cells resistant to methotrexate.

ABSTRACT:

Background

The need for an integrated view of data obtained from high-throughput technologies gave rise to network analyses. These are especially useful to rationalize how external perturbations propagate through the expression of genes. To address this issue in the case of drug resistance, we constructed biological association networks of genes differentially expressed in cell lines resistant to methotrexate (MTX).

Methods

Seven cell lines representative of different types of cancer, including colon cancer (HT29 and Caco2), breast cancer (MCF-7 and MDA-MB-468), pancreatic cancer (MIA PaCa-2), erythroblastic leukemia (K562) and osteosarcoma (Saos-2), were used. The differential expression pattern between sensitive and MTX-resistant cells was determined by whole human genome

SUBMITTER: Selga E 

PROVIDER: S-EPMC2768990 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature