Global gene expression patterns in the post-pneumonectomy lung of adult mice.

ABSTRACT:

Background

Adult mice have a remarkable capacity to regenerate functional alveoli following either lung resection or injury that exceeds the regenerative capacity observed in larger adult mammals. The molecular basis for this unique capability in mice is largely unknown. We examined the transcriptomic responses to single lung pneumonectomy in adult mice in order to elucidate prospective molecular signaling mechanisms used in this species during lung regeneration.

Methods

Unilateral left pneumonectomy or sham thoracotomy was performed under general anesthesia (n = 8 mice per group for each of the four time points). Total RNA was isolated from the remaining lung tissue at four time points post-surgery (6 hours, 1 day, 3 days, 7 days) and analyzed using microarray technology.

SUBMITTER: Paxson JA 

PROVIDER: S-EPMC2770038 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature