Cloning of Bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes.

ABSTRACT:

Background

Bovine herpesviruses type 1 (BoHV1) and type 5 (BoHV5) are two closely related pathogens of cattle. The identity of the two viruses on the amino acid level averages 82%. Despite their high antigenetic similarities the two pathogens induce distinctive clinical signs. BoHV1 causes respiratory and genital tract infections while BoHV5 leads to severe encephalitis in calves.

Findings

The viral genomes of BoHV1 and BoHV5 were cloned as infectious bacterial artificial chromosomes (BACs). First, recombinant viruses carrying the genetic elements for propagation in bacteria were generated. Second, DNA from these recombinant viruses were transferred into prokaryotic cells. Third, DNA from these bacteria were transferred into eukaryotic cells. Progeny viruses from BAC transfe

SUBMITTER: Gabev E 

PROVIDER: S-EPMC2770474 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature