IQuantitator: a tool for protein expression inference using iTRAQ.

ABSTRACT:

Background

Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) [Applied Biosystems] have seen increased application in differential protein expression analysis. To facilitate the growing need to analyze iTRAQ data, especially for cases involving multiple iTRAQ experiments, we have developed a modeling approach, statistical methods, and tools for estimating the relative changes in protein expression under various treatments and experimental conditions.

Results

This modeling approach provides a unified analysis of data from multiple iTRAQ experiments and links the observed quantity (reporter ion peak area) to the experiment design and the calculated quantity of interest (treatment-dependent protein and peptide fold change) through an additive model under log transform

SUBMITTER: Schwacke JH 

PROVIDER: S-EPMC2770557 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature