BFAST: an alignment tool for large scale genome resequencing.

ABSTRACT:

Background

The new generation of massively parallel DNA sequencers, combined with the challenge of whole human genome resequencing, result in the need for rapid and accurate alignment of billions of short DNA sequence reads to a large reference genome. Speed is obviously of great importance, but equally important is maintaining alignment accuracy of short reads, in the 25-100 base range, in the presence of errors and true biological variation.

Methodology

We introduce a new algorithm specifically optimized for this task, as well as a freely available implementation, BFAST, which can align data produced by any of current sequencing platforms, allows for user-customizable levels of speed and accuracy, supports paired end data, and provides for efficient parallel and multi-thre

SUBMITTER: Homer N 

PROVIDER: S-EPMC2770639 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature