Yeast genome analysis identifies chromosomal translocation, gene conversion events and several sites of Ty element insertion.

ABSTRACT: Paired end mapping of chromosomal fragments has been used in human cells to identify numerous structural variations in chromosomes of individuals and of cancer cell lines; however, the molecular, biological and bioinformatics methods for this technology are still in development. Here, we present a parallel bioinformatics approach to analyze chromosomal paired-end tag (ChromPET) sequence data and demonstrate its application in identifying gene rearrangements in the model organism Saccharomyces cerevisiae. We detected several expected events, including a chromosomal rearrangement of the nonessential arm of chromosome V induced by selective pressure, rearrangements introduced during strain construction and gene conversion at the MAT locus. In addition, we discovered several unannotated Ty ele

SUBMITTER: Shibata Y 

PROVIDER: S-EPMC2770650 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature