Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation.

ABSTRACT: Activation of the type I interferon (IFN) pathway by small interfering RNA (siRNA) is a major contributor to the off-target effects of RNA interference in mammalian cells. While IFN induction complicates gene function studies, immunostimulation by siRNAs may be beneficial in certain therapeutic settings. Various forms of siRNA, meeting different compositional and structural requirements, have been reported to trigger IFN activation. The consensus is that intracellularly expressed short-hairpin RNAs (shRNAs) are less prone to IFN activation because they are not detected by the cell-surface receptors. In particular, lentiviral vector-mediated transduction of shRNAs has been reported to avoid IFN response. Here we identify a shRNA that potently activates the IFN pathway in human cells in a se

SUBMITTER: Kenworthy R 

PROVIDER: S-EPMC2770676 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature