Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei.

ABSTRACT:

Background

Trypanosoma brucei, the causative agent of African sleeping sickness, undergoes a complex developmental cycle that takes place in mammalian and insect hosts and is accompanied by changes in metabolism and cellular morphology. While differences in mRNA expression have been described for many genes, genome-wide expression analyses have been largely lacking. Trypanosomatids represent a unique case in eukaryotes in that they transcribe protein-coding genes as large polycistronic units, and rarely regulate gene expression at the level of transcription initiation.

Results

Here we present a comprehensive analysis of mRNA expression in several stages of parasite development. Utilizing microarrays that have multiple copies of multiple probes for each gene, we were able to

SUBMITTER: Jensen BC 

PROVIDER: S-EPMC2771046 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature