A multifunctional, synthetic Gaussia princeps luciferase reporter for live imaging of Candida albicans infections.

ABSTRACT: Real-time monitoring of the spatial and temporal progression of infection/gene expression in animals will contribute greatly to our understanding of host-pathogen interactions while reducing the number of animals required to generate statistically significant data sets. Sensitive in vivo imaging technologies can detect low levels of light emitted from luciferase reporters in vivo, but the existing reporters are not optimal for fungal infections. Therefore, our aim was to develop a novel reporter system for imaging Candida albicans infections that overcomes the limitations of current luciferase reporters for this major fungal pathogen. This luciferase reporter was constructed by fusing a synthetic, codon-optimized version of the Gaussia princeps luciferase gene to C. albicans PGA59, which e

SUBMITTER: Enjalbert B 

PROVIDER: S-EPMC2772526 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature