Design and validation of real-time reverse transcription-PCR assays for detection of pandemic (H1N1) 2009 virus.

ABSTRACT: Tracking novel influenza viruses which have the potential to cause pandemics, such as the pandemic (H1N1) 2009 virus, is a public health priority. Pandemic (H1N1) 2009 virus was first identified in Mexico in April 2009 and spread worldwide over a short period of time. Well-validated diagnostic tools that are rapid, sensitive, and specific for the detection and tracking of this virus are needed. Three real-time reverse transcription PCR (RT-PCR) assays for the amplification and detection of pandemic (H1N1) 2009 virus were developed, and their performance characteristics were compared with those of other published diagnostic assays. Thirty-nine samples confirmed to be positive for pandemic (H1N1) 2009 virus from Alberta, Canada, and six additional samples that were positive for influenza A v

SUBMITTER: Pabbaraju K 

PROVIDER: S-EPMC2772599 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature