Cryo-electron microscopy structure of an adenovirus-integrin complex indicates conformational changes in both penton base and integrin.

ABSTRACT: A structure of adenovirus type 12 (HAdV12) complexed with a soluble form of integrin alphavbeta5 was determined by cryo-electron microscopy (cryoEM) image reconstruction. Subnanometer resolution (8 A) was achieved for the icosahedral capsid with moderate resolution (27 A) for integrin density above each penton base. Modeling with alphavbeta3 and alpha(IIb)beta3 crystal structures indicates that a maximum of four integrins fit over the pentameric penton base. The close spacing (approximately 60 A) of the RGD protrusions on penton base precludes integrin binding in the same orientation to neighboring RGD sites. Flexible penton-base RGD loops and incoherent averaging of bound integrin molecules explain the moderate resolution observed for the integrin density. A model with four integrins boun

SUBMITTER: Lindert S 

PROVIDER: S-EPMC2772687 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature