Gene regulation and epigenetic remodeling in murine embryonic stem cells by c-Myc.

ABSTRACT:

Background

The Myc oncoprotein, a transcriptional regulator involved in the etiology of many different tumor types, has been demonstrated to play an important role in the functions of embryonic stem (ES) cells. Nonetheless, it is still unclear as to whether Myc has unique target and functions in ES cells.

Methodology/principal findings

To elucidate the role of c-Myc in murine ES cells, we mapped its genomic binding sites by chromatin-immunoprecipitation combined with DNA microarrays (ChIP-chip). In addition to previously identified targets we identified genes involved in pluripotency, early development, and chromatin modification/structure that are bound and regulated by c-Myc in murine ES cells. Myc also binds and regulates loci previously identified as Polycomb (PcG) targe

SUBMITTER: Lin CH 

PROVIDER: S-EPMC2773118 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature