Gene expression profiling: canonical molecular changes and clinicopathological features in sporadic colorectal cancers.

ABSTRACT:

Aim

To investigate alternative or subordinate pathways involved in colorectal tumorigenesis and tumor growth, possibly determining at-risk populations and predicting responses to treatment.

Methods

Using microarray gene-expression analysis, we analyzed patterns of gene expression relative to canonical molecular changes and clinicopathological features in 84 sporadic colorectal cancer patients, standardized by tumor location. Subsets of differentially expressed genes were confirmed by real-time reverse-transcript polymerase chain reaction (RT-PCR).

Results

The largest number of genes identified as being differentially expressed was by tumor location, and the next largest number by lymphovascular or neural invasion of tumor cells and by mismatch repair (MMR) defects. Am

SUBMITTER: Kim JC 

PROVIDER: S-EPMC2773308 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature