A new approach for quantitative phosphoproteomic dissection of signaling pathways applied to T cell receptor activation.

ABSTRACT: Reversible protein phosphorylation plays a pivotal role in the regulation of cellular signaling pathways. Current approaches in phosphoproteomics focus on analysis of the global phosphoproteome in a single cellular state or of receptor stimulation time course experiments, often with a restricted number of time points. Although these studies have provided some insights into newly discovered phosphorylation sites that may be involved in pathways, they alone do not provide enough information to make precise predictions of the placement of individual phosphorylation events within a signaling pathway. Protein disruption and site-directed mutagenesis are essential to clearly define the precise biological roles of the hundreds of newly discovered phosphorylation sites uncovered in modern proteomi

SUBMITTER: Nguyen V 

PROVIDER: S-EPMC2773711 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature