Analysis of chromosomal aberration (1, 3, and 8) and association of microRNAs in uveal melanoma.

ABSTRACT:

Purpose

Uveal melanoma is the most common intraocular primary tumor, involving iris, ciliary body and choroid. More than 90% of the patients develop hepatic metastasis with an average survival time of 7 months. We have used formalin fixed paraffin embedded sections to validate the presence of monosomy 3, an accurate predictor of metastasis, chromosome 8 isochromosome (8q22), and 1p36 deletion. This study also tested the presence of oncomirs in uveal melanoma samples by microRNA (miRNA) expression profiling.

Methods

Chromogenic in situ hybridization (CISH) was performed in formalin fixed, paraffin embedded sections of uveal melanoma to analyze chromosome 1, 3, and 8 aberrations (n=60). MicroRNA (miRNA) expression profiling was done on paraffin sections of invasive tumor with

SUBMITTER: Radhakrishnan A 

PROVIDER: S-EPMC2773734 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature