Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersonii.

ABSTRACT:

Background

Exposure of cells to environmental stress conditions can lead to the interruption of several intracellular processes, in particular those performed by macromolecular complexes such as the spliceosome.

Results

During nucleotide sequencing of cDNA libraries constructed using RNA isolated from B. emersonii cells submitted to heat shock and cadmium stress, a large number of ESTs with retained introns was observed. Among the 6,350 ESTs obtained through sequencing of stress cDNA libraries, 181 ESTs presented putative introns (2.9%), while sequencing of cDNA libraries from unstressed B. emersonii cells revealed only 0.2% of ESTs containing introns. These data indicate an enrichment of ESTs with introns in B. emersonii stress cDNA libraries. Among the 85 genes correspondi

SUBMITTER: Georg RC 

PROVIDER: S-EPMC2773782 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature