Direct calibration of PICKY-designed microarrays.

ABSTRACT:

Background

Few microarrays have been quantitatively calibrated to identify optimal hybridization conditions because it is difficult to precisely determine the hybridization characteristics of a microarray using biologically variable cDNA samples.

Results

Using synthesized samples with known concentrations of specific oligonucleotides, a series of microarray experiments was conducted to evaluate microarrays designed by PICKY, an oligo microarray design software tool, and to test a direct microarray calibration method based on the PICKY-predicted, thermodynamically closest nontarget information. The complete set of microarray experiment results is archived in the GEO database with series accession number GSE14717. Additional data files and Perl programs described in this paper

SUBMITTER: Chou HH 

PROVIDER: S-EPMC2773793 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature