Matrix metalloproteinase-14 mediates a phenotypic shift in the airways to increase mucin production.

ABSTRACT:

Rationale

Induced mainly by cigarette smoking, chronic obstructive pulmonary disease (COPD) is a global public health problem characterized by progressive difficulty in breathing and increased mucin production. Previously, we reported that acrolein levels found in COPD sputum could activate matrix metalloproteinase-9 (MMP9).

Objectives

To determine whether acrolein increases expression and activity of MMP14, a critical membrane-bound endopeptidase that can initial a MMP-activation cascade.

Methods

MMP14 activity and adduct formation were measured following direct acrolein treatment. MMP14 expression and activity was measured in human airway epithelial cells. MMP14 immunohistochemistry was performed with COPD tissue, and in acrolein- or tobacco-exposed mice.

Measure

SUBMITTER: Deshmukh HS 

PROVIDER: S-EPMC2773913 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature