A functional analysis of the CREB signaling pathway using HaloCHIP-chip and high throughput reporter assays.

ABSTRACT:

Background

Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. To measure CREB binding, we used HaloCHIP, an antibody-free alternative to the ChIP method that utilizes the HaloTag fusion protein, and also high-throughput promoter-luciferase reporter assays, which provide rapid and quantitative screening of promoters for transcriptional activation or repression in living cells.

Results

In analy

SUBMITTER: Hartzell DD 

PROVIDER: S-EPMC2774331 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature