A microarray analysis of retinal transcripts that are controlled by image contrast in mice.

ABSTRACT:

Purpose

The development of myopia is controlled by still largely unknown retinal signals. The aim of this study was to investigate the changes in retinal mRNA expression after different periods of visual deprivation in mice, while controlling for retinal illuminance.

Methods

Each group consisted of three male C57BL/6 mice. Treatment periods were 30 min, 4 h, and 6+6 h. High spatial frequencies were filtered from the retinal image by frosted diffusers over one eye while the fellow eyes were covered by clear neutral density (ND) filters that exhibited similar light attenuating properties (0.1 log units) as the diffusers. For the final 30 min of the respective treatment period mice were individually placed in a clear Perspex cylinder that was positioned in the center of a rotat

SUBMITTER: Brand C 

PROVIDER: S-EPMC2774458 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature