Infrared multiphoton dissociation of peptide cations in a dual pressure linear ion trap mass spectrometer.

ABSTRACT: A dual pressure linear ion trap mass spectrometer was modified to permit infrared multiphoton dissociation (IRMPD) in each of the two cells-the first a high pressure cell operated at nominally 5 x 10(-3) Torr and the second a low pressure cell operated at nominally 3 x 10(-4) Torr. When IRMPD was performed in the high pressure cell, most peptide ions did not undergo significant photodissociation; however, in the low pressure cell peptide cations were efficiently dissociated with less than 25 ms of IR irradiation regardless of charge state. IRMPD of peptide cations allowed the detection of low m/z product ions including the y(1) fragments and immonium ions which are not typically observed by ion trap collision induced dissociation (CID). Photodissociation efficiencies of approximately 100%

SUBMITTER: Gardner MW 

PROVIDER: S-EPMC2774747 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature