Cooperation of decay-accelerating factor and membrane cofactor protein in regulating survival of human cervical cancer cells.

ABSTRACT:

Background

Decay-accelerating factor (DAF) and membrane cofactor protein (MCP) are the key molecules involved in cell protection against autologus complement, which restricts the action of complement at critical stages of the cascade reaction. The cooperative effect of DAF and MCP on the survival of human cervical cancer cell (ME180) has not been demonstrated.

Methods

In this study we applied, for the first time, short hairpin RNA (shRNA) to knock down the expression of the DAF and MCP with the aim of exploiting complement more effectively for tumor cell damage. Meanwhile, we investigated the cooperative effects of DAF and MCP on the viability and migration, moreover the proliferation of ME180 cell.

Results

The results showed that shRNA inhibition of DAF and MCP expre

SUBMITTER: Gao LJ 

PROVIDER: S-EPMC2774863 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature